densitometry analysis software alphalmager Search Results


alphaimager software 290  (Alpha Innotech)
86
Alpha Innotech alphaimager software 290
Alphaimager Software 290, supplied by Alpha Innotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/densitometry+analysis+software+alphalmager/pm28971894-117-10-16?v=Alpha+Innotech
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alphaimager software 290 - by Bioz Stars, 2026-08
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alphaimager 2200  (Alpha Innotech)
86
Alpha Innotech alphaimager 2200
Alphaimager 2200, supplied by Alpha Innotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/densitometry+analysis+software+alphalmager/pm17978486-67-8-10?v=Alpha+Innotech
Average 86 stars, based on 1 article reviews
alphaimager 2200 - by Bioz Stars, 2026-08
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alphaimager ep analysis software  (Cell Biosciences Inc)
90
Cell Biosciences Inc alphaimager ep analysis software
Alphaimager Ep Analysis Software, supplied by Cell Biosciences Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/densitometry+analysis+software+alphalmager/pmc09587155-85-5-9?v=Cell+Biosciences+Inc
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alphaimager ep analysis software - by Bioz Stars, 2026-08
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densitometry analysis software  (Alpha Innotech)
86
Alpha Innotech densitometry analysis software
Figure 2. Reduced homo-dimerization of pathogenic DJ-1 mutants. (A) Cross-linking of DJ-1 homo-dimers. Equivalent soluble lysates from SH-SY5Y cells transfected with myc-tagged DJ-1 (WT or L166P) were treated with increasing concentrations of DSS and myc-tagged or endogenous DJ-1 was detected by WB with anti-myc (upper panel) or anti-DJ-1 (N) (lower panel) antibodies, respectively. The corresponding position of myc-tagged (Myc) or endogenous (endo) DJ-1 monomers and homo-dimers are indicated. (B) Reduced cross-linking of pathogenic DJ-1 mutants. Equivalent lysates from SH-SY5Y cells trans- fected with myc-tagged WT or mutant (L166P, M26I, A104T, D149A or R98Q) DJ-1 were treated with 5 mM DSS and homo-dimer formation was assessed by WB with anti-myc antibody. (C) Quantification of homo-dimer levels and normalization to monomer levels from (B) by <t>densitometry</t> reveals a significant reduction in the homo-dimerization of pathogenic DJ-1 mutants. DJ-1 dimer–monomer ratios are expressed as a percentage (%) of WT levels, and bars represent the mean + SE of three independent experiments, P , 0.005 when compared with WT levels (Student’s t-test). (D) Parkin fails to cross-link to pathogenic DJ-1 mutants. SH-SY5Y cells were co-transfected with myc-tagged WT or mutant (L166P, M26I, A104T, D149A or R98Q) DJ-1 or control plasmid, together with FLAG-tagged parkin. Equivalent lysates were treated with 5 mM DSS and then subjected to IP with anti-myc antibody. IP and input lysates were analyzed by WB with anti-FLAG and anti-myc antibodies. NS denotes non-specific band detected with anti-myc antibody. Molecular weight markers are indicated in kDa. All experiments were replicated three times with similar results.
Densitometry Analysis Software, supplied by Alpha Innotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/densitometry+analysis+software+alphalmager/pm15525661-308-7-11?v=Alpha+Innotech
Average 86 stars, based on 1 article reviews
densitometry analysis software - by Bioz Stars, 2026-08
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alphaimager software  (Alpha Innotech)
86
Alpha Innotech alphaimager software
Figure 2. Reduced homo-dimerization of pathogenic DJ-1 mutants. (A) Cross-linking of DJ-1 homo-dimers. Equivalent soluble lysates from SH-SY5Y cells transfected with myc-tagged DJ-1 (WT or L166P) were treated with increasing concentrations of DSS and myc-tagged or endogenous DJ-1 was detected by WB with anti-myc (upper panel) or anti-DJ-1 (N) (lower panel) antibodies, respectively. The corresponding position of myc-tagged (Myc) or endogenous (endo) DJ-1 monomers and homo-dimers are indicated. (B) Reduced cross-linking of pathogenic DJ-1 mutants. Equivalent lysates from SH-SY5Y cells trans- fected with myc-tagged WT or mutant (L166P, M26I, A104T, D149A or R98Q) DJ-1 were treated with 5 mM DSS and homo-dimer formation was assessed by WB with anti-myc antibody. (C) Quantification of homo-dimer levels and normalization to monomer levels from (B) by <t>densitometry</t> reveals a significant reduction in the homo-dimerization of pathogenic DJ-1 mutants. DJ-1 dimer–monomer ratios are expressed as a percentage (%) of WT levels, and bars represent the mean + SE of three independent experiments, P , 0.005 when compared with WT levels (Student’s t-test). (D) Parkin fails to cross-link to pathogenic DJ-1 mutants. SH-SY5Y cells were co-transfected with myc-tagged WT or mutant (L166P, M26I, A104T, D149A or R98Q) DJ-1 or control plasmid, together with FLAG-tagged parkin. Equivalent lysates were treated with 5 mM DSS and then subjected to IP with anti-myc antibody. IP and input lysates were analyzed by WB with anti-FLAG and anti-myc antibodies. NS denotes non-specific band detected with anti-myc antibody. Molecular weight markers are indicated in kDa. All experiments were replicated three times with similar results.
Alphaimager Software, supplied by Alpha Innotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/densitometry+analysis+software+alphalmager/pmc12751842-77-5-7?v=Alpha+Innotech
Average 86 stars, based on 1 article reviews
alphaimager software - by Bioz Stars, 2026-08
86/100 stars
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densitometric analysis  (Alpha Innotech)
86
Alpha Innotech densitometric analysis
Figure 2. Reduced homo-dimerization of pathogenic DJ-1 mutants. (A) Cross-linking of DJ-1 homo-dimers. Equivalent soluble lysates from SH-SY5Y cells transfected with myc-tagged DJ-1 (WT or L166P) were treated with increasing concentrations of DSS and myc-tagged or endogenous DJ-1 was detected by WB with anti-myc (upper panel) or anti-DJ-1 (N) (lower panel) antibodies, respectively. The corresponding position of myc-tagged (Myc) or endogenous (endo) DJ-1 monomers and homo-dimers are indicated. (B) Reduced cross-linking of pathogenic DJ-1 mutants. Equivalent lysates from SH-SY5Y cells trans- fected with myc-tagged WT or mutant (L166P, M26I, A104T, D149A or R98Q) DJ-1 were treated with 5 mM DSS and homo-dimer formation was assessed by WB with anti-myc antibody. (C) Quantification of homo-dimer levels and normalization to monomer levels from (B) by <t>densitometry</t> reveals a significant reduction in the homo-dimerization of pathogenic DJ-1 mutants. DJ-1 dimer–monomer ratios are expressed as a percentage (%) of WT levels, and bars represent the mean + SE of three independent experiments, P , 0.005 when compared with WT levels (Student’s t-test). (D) Parkin fails to cross-link to pathogenic DJ-1 mutants. SH-SY5Y cells were co-transfected with myc-tagged WT or mutant (L166P, M26I, A104T, D149A or R98Q) DJ-1 or control plasmid, together with FLAG-tagged parkin. Equivalent lysates were treated with 5 mM DSS and then subjected to IP with anti-myc antibody. IP and input lysates were analyzed by WB with anti-FLAG and anti-myc antibodies. NS denotes non-specific band detected with anti-myc antibody. Molecular weight markers are indicated in kDa. All experiments were replicated three times with similar results.
Densitometric Analysis, supplied by Alpha Innotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/densitometry+analysis+software+alphalmager/10__3168_slash_jds__s0022___0302_ascii40_04_ascii41_73462___1-90-5-8?v=Alpha+Innotech
Average 86 stars, based on 1 article reviews
densitometric analysis - by Bioz Stars, 2026-08
86/100 stars
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Image Search Results


Figure 2. Reduced homo-dimerization of pathogenic DJ-1 mutants. (A) Cross-linking of DJ-1 homo-dimers. Equivalent soluble lysates from SH-SY5Y cells transfected with myc-tagged DJ-1 (WT or L166P) were treated with increasing concentrations of DSS and myc-tagged or endogenous DJ-1 was detected by WB with anti-myc (upper panel) or anti-DJ-1 (N) (lower panel) antibodies, respectively. The corresponding position of myc-tagged (Myc) or endogenous (endo) DJ-1 monomers and homo-dimers are indicated. (B) Reduced cross-linking of pathogenic DJ-1 mutants. Equivalent lysates from SH-SY5Y cells trans- fected with myc-tagged WT or mutant (L166P, M26I, A104T, D149A or R98Q) DJ-1 were treated with 5 mM DSS and homo-dimer formation was assessed by WB with anti-myc antibody. (C) Quantification of homo-dimer levels and normalization to monomer levels from (B) by densitometry reveals a significant reduction in the homo-dimerization of pathogenic DJ-1 mutants. DJ-1 dimer–monomer ratios are expressed as a percentage (%) of WT levels, and bars represent the mean + SE of three independent experiments, P , 0.005 when compared with WT levels (Student’s t-test). (D) Parkin fails to cross-link to pathogenic DJ-1 mutants. SH-SY5Y cells were co-transfected with myc-tagged WT or mutant (L166P, M26I, A104T, D149A or R98Q) DJ-1 or control plasmid, together with FLAG-tagged parkin. Equivalent lysates were treated with 5 mM DSS and then subjected to IP with anti-myc antibody. IP and input lysates were analyzed by WB with anti-FLAG and anti-myc antibodies. NS denotes non-specific band detected with anti-myc antibody. Molecular weight markers are indicated in kDa. All experiments were replicated three times with similar results.

Journal: Human molecular genetics

Article Title: Association of DJ-1 and parkin mediated by pathogenic DJ-1 mutations and oxidative stress.

doi: 10.1093/hmg/ddi007

Figure Lengend Snippet: Figure 2. Reduced homo-dimerization of pathogenic DJ-1 mutants. (A) Cross-linking of DJ-1 homo-dimers. Equivalent soluble lysates from SH-SY5Y cells transfected with myc-tagged DJ-1 (WT or L166P) were treated with increasing concentrations of DSS and myc-tagged or endogenous DJ-1 was detected by WB with anti-myc (upper panel) or anti-DJ-1 (N) (lower panel) antibodies, respectively. The corresponding position of myc-tagged (Myc) or endogenous (endo) DJ-1 monomers and homo-dimers are indicated. (B) Reduced cross-linking of pathogenic DJ-1 mutants. Equivalent lysates from SH-SY5Y cells trans- fected with myc-tagged WT or mutant (L166P, M26I, A104T, D149A or R98Q) DJ-1 were treated with 5 mM DSS and homo-dimer formation was assessed by WB with anti-myc antibody. (C) Quantification of homo-dimer levels and normalization to monomer levels from (B) by densitometry reveals a significant reduction in the homo-dimerization of pathogenic DJ-1 mutants. DJ-1 dimer–monomer ratios are expressed as a percentage (%) of WT levels, and bars represent the mean + SE of three independent experiments, P , 0.005 when compared with WT levels (Student’s t-test). (D) Parkin fails to cross-link to pathogenic DJ-1 mutants. SH-SY5Y cells were co-transfected with myc-tagged WT or mutant (L166P, M26I, A104T, D149A or R98Q) DJ-1 or control plasmid, together with FLAG-tagged parkin. Equivalent lysates were treated with 5 mM DSS and then subjected to IP with anti-myc antibody. IP and input lysates were analyzed by WB with anti-FLAG and anti-myc antibodies. NS denotes non-specific band detected with anti-myc antibody. Molecular weight markers are indicated in kDa. All experiments were replicated three times with similar results.

Article Snippet: Quantitation of protein expression was performed using densitometry analysis software (AlphaImager, Alpha Innotech Corp.) and data were analyzed by Student’s t-test to determine differences between groups.

Techniques: Transfection, Mutagenesis, Control, Plasmid Preparation, Molecular Weight